Plasmid Preparation:Article Title: A tripartite chemogenetic fluorescent reporter for imaging ternary protein interactions
Article Snippet: .. The plasmid pAG1240 allowing the mammalian expression of TOM20-mCherry- C FRB-pFAST 1-98 was constructed by replacing the Lyn11 sequence by TOM20 sequence, amplified from the Addgene vector #171461 Tom20-CR coding for TOM20-ECFP-FRB , in the vector pAG1223. .. The plasmid pAG1225 allowing the mammalian expression of TOM20-FKBP-pFAST 114-125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 114-125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for TOM20-ECFP-FRB .
Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
Article Snippet: .. The plasmid pAG1240 allowing the mammalian expression of Tom20-mCherry- C FRB-pFAST 1-98 was constructed by replacing the Lyn11 sequence by Tom20 sequence, amplified from the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB , in the vector pAG1223. .. The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB .
Expressing:Article Title: A tripartite chemogenetic fluorescent reporter for imaging ternary protein interactions
Article Snippet: .. The plasmid pAG1240 allowing the mammalian expression of TOM20-mCherry- C FRB-pFAST 1-98 was constructed by replacing the Lyn11 sequence by TOM20 sequence, amplified from the Addgene vector #171461 Tom20-CR coding for TOM20-ECFP-FRB , in the vector pAG1223. .. The plasmid pAG1225 allowing the mammalian expression of TOM20-FKBP-pFAST 114-125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 114-125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for TOM20-ECFP-FRB .
Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
Article Snippet: .. The plasmid pAG1240 allowing the mammalian expression of Tom20-mCherry- C FRB-pFAST 1-98 was constructed by replacing the Lyn11 sequence by Tom20 sequence, amplified from the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB , in the vector pAG1223. .. The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB .
Construct:Article Title: A tripartite chemogenetic fluorescent reporter for imaging ternary protein interactions
Article Snippet: .. The plasmid pAG1240 allowing the mammalian expression of TOM20-mCherry- C FRB-pFAST 1-98 was constructed by replacing the Lyn11 sequence by TOM20 sequence, amplified from the Addgene vector #171461 Tom20-CR coding for TOM20-ECFP-FRB , in the vector pAG1223. .. The plasmid pAG1225 allowing the mammalian expression of TOM20-FKBP-pFAST 114-125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 114-125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for TOM20-ECFP-FRB .
Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
Article Snippet: .. The plasmid pAG1240 allowing the mammalian expression of Tom20-mCherry- C FRB-pFAST 1-98 was constructed by replacing the Lyn11 sequence by Tom20 sequence, amplified from the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB , in the vector pAG1223. .. The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB .
Sequencing:Article Title: A tripartite chemogenetic fluorescent reporter for imaging ternary protein interactions
Article Snippet: .. The plasmid pAG1240 allowing the mammalian expression of TOM20-mCherry- C FRB-pFAST 1-98 was constructed by replacing the Lyn11 sequence by TOM20 sequence, amplified from the Addgene vector #171461 Tom20-CR coding for TOM20-ECFP-FRB , in the vector pAG1223. .. The plasmid pAG1225 allowing the mammalian expression of TOM20-FKBP-pFAST 114-125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 114-125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for TOM20-ECFP-FRB .
Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
Article Snippet: .. The plasmid pAG1240 allowing the mammalian expression of Tom20-mCherry- C FRB-pFAST 1-98 was constructed by replacing the Lyn11 sequence by Tom20 sequence, amplified from the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB , in the vector pAG1223. .. The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB .
Amplification:Article Title: A tripartite chemogenetic fluorescent reporter for imaging ternary protein interactions
Article Snippet: .. The plasmid pAG1240 allowing the mammalian expression of TOM20-mCherry- C FRB-pFAST 1-98 was constructed by replacing the Lyn11 sequence by TOM20 sequence, amplified from the Addgene vector #171461 Tom20-CR coding for TOM20-ECFP-FRB , in the vector pAG1223. .. The plasmid pAG1225 allowing the mammalian expression of TOM20-FKBP-pFAST 114-125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 114-125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for TOM20-ECFP-FRB .
Article Title: Design and evaluation of a tripartite chemogenetic fluorescent reporter for visualizing ternary protein complexes
Article Snippet: .. The plasmid pAG1240 allowing the mammalian expression of Tom20-mCherry- C FRB-pFAST 1-98 was constructed by replacing the Lyn11 sequence by Tom20 sequence, amplified from the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB , in the vector pAG1223. .. The plasmid pAG1225bis allowing the mammalian expression of Tom20-FKBP-pFAST 115–125 was generated by replacing the sequence encoding ECFP-FRB by the FKBP-pFAST 115–125 sequence, amplified from pAG1151, in the Addgene vector #171461 Tom20-CR coding for Tom20-ECFP-FRB .
|
Addgene inc is a verified supplier 